Tlr4^em1Svog
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tlr4^em1Svog
• Name: toll-like receptor 4; endonuclease-mediated mutation 1, Stefanie N Vogel
• MGI ID: MGI:7530030
• Synonyms: TLR4-SNP D298G/N397I
• Gene: Tlr4 Location: Chr4:66745788-66765338 bp, + strand Genetic Position: Chr4, 34.66 cM
• Alliance: Tlr4^em1Svog page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: Aspartic acid codon 298 (GAT) in exon 3 was changed to glycine (GGT) (p.298G) and asparagine codon 397 (AAC) in exon 3 to isoleucine (ATC) (p.N397I) using sgRNAs and ssODN templates with CRISPR/Cas9 technology. The mutations replicate the human p.D299G (c.896A>G, rs4986790) and p.T399I (c.1196C>T, rs4986791) mutations associated with LPS hyporesponsiveness and differential susceptibility to many infectious and non-infectious diseases. (J:303648)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlr4 Mutation: 96 strains or lines available

References

• Original: J:303648 Richard K, et al., A mouse model of human TLR4 D299G/T399I SNPs reveals mechanisms of altered LPS and pathogen responses. J Exp Med. 2021 Feb 1;218(2)
• All: 3 reference(s)