Raf1^tm1Bbd
Targeted Allele Detail

Summary

• Symbol: Raf1^tm1Bbd
• Name: Raf1 proto-oncogene, serine/threonine kinase; targeted mutation 1, Mariano Barbacid
• MGI ID: MGI:7529717
• Synonyms: RAF1^LMLK375M
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^tm1Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:303935
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site, a minigene consisting of the cDNA sequence for exons 11-17, a STOP cassette, a neomycin resistance gene cassette and a second loxP site were inserted into intron 10 and endogenous exon 11 was modified to change lysine codon 375 (AAG) to methionine (ATG) (p.K375M). This allele expresses the wildtype transcript and only after Cre-mediated deletion of the minigene-STOP-neo cassette will it express the mutated transcript. The mutation renders the encoded protein kinase-dead. (J:303935)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Raf1 Mutation: 116 strains or lines available

References

• Original: J:303935 Sanclemente M, et al., RAF1 kinase activity is dispensable for KRAS/p53 mutant lung tumor progression. Cancer Cell. 2021 Mar 8;39(3):294-296
• All: 1 reference(s)