Cib2^em1Mll
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cib2^em1Mll
• Name: calcium and integrin binding family member 2; endonuclease-mediated mutation 1, Ulrich Muller
• MGI ID: MGI:7529458
• Synonyms: Cib2-E64D, Cib2^E64D
• Gene: Cib2 Location: Chr9:54452078-54467502 bp, - strand Genetic Position: Chr9, 29.7 cM
• Alliance: Cib2^em1Mll page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Single point mutation
• Mutation details: Glutamic acid codon 64 (GAG) in exon 3 was changed to aspartic acid (GAC) (p.E64D) using crRNA, tracrRNA and an ssODN template with CRISPR/Cas9 technology. The mutation affects mechanoelectrical transduction (MET) by cochlear hair cells and causes deafness. (J:307668)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cib2 Mutation: 15 strains or lines available

References

• Original: J:307668 Liang X, et al., CIB2 and CIB3 are auxiliary subunits of the mechanotransduction channel of hair cells. Neuron. 2021 Jul 7;109(13):2131-2149.e15
• All: 1 reference(s)