Gt(ROSA)26Sor^{tm1(CAG-CHRM4*,-mKateT)Dschm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-CHRM4*,-mKateT)Dschm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Dietmar Schmitz
• MGI ID: MGI:7528984
• Synonyms: Rosa26-FLEX-hM4D
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-CHRM4*,-mKateT)Dschm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304133
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Recombination-mediated cassette exchange (RMCE) inserted a cre-conditional FLEX (flip-excision) cassette with inserted a CAG promoter in front of the FLEX-cassette containing the coding sequence of hM4Di-mKateT was inserted into the FLEX cassette in reverse orientation to the promoter and a rabbit globulin polyA cassette including stop codons in every reading frame downstream of the FLEX cassette, in the same direction as hM4Di. The hM4D ORF is followed by a 6x His Tag and a V5 tag; however, these could not be detected by immunohistochemistry on PFA fixed tissue. (J:304133)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:304133 de Filippo R, et al., Somatostatin interneurons activated by 5-HT2A receptor suppress slow oscillations in medial entorhinal cortex. Elife. 2021 Mar 31;10:e66960
• All: 2 reference(s)