Xrcc6^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Xrcc6^{tm1c(KOMP)Mbp}
• Name: X-ray repair cross complementing 6; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:7526100
• Synonyms: Ku70^F
• Gene: Xrcc6 Location: Chr15:81872036-81924286 bp, + strand Genetic Position: Chr15, 38.33 cM, cytoband E-F
• Alliance: Xrcc6^{tm1c(KOMP)Mbp} page
• IMPC: Xrcc6 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340115
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 81911001 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 81911050. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:340115)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xrcc6 Mutation: 64 strains or lines available

References

• Original: J:340115 Myler LR, et al., DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres. Nat Struct Mol Biol. 2023 Aug 31
• All: 1 reference(s)