Syt7^tm3.2Sud
Targeted Allele Detail

Summary

• Symbol: Syt7^tm3.2Sud
• Name: synaptotagmin VII; targeted mutation 3.2, Thomas C Sudhof
• MGI ID: MGI:7525660
• Synonyms: 7SC
• Gene: Syt7 Location: Chr19:10366454-10430544 bp, + strand Genetic Position: Chr19, 6.58 cM, cytoband B
• Alliance: Syt7^tm3.2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249264
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using homologous recombination, exon 2 of the mouse Syt7 gene that encodes the transmembrane region was modified to introduce a haemagglutinin (HA) tag between the Syt7 protein N-terminal and the transmembrane region; in addition, a loxP site was introduced into the 5 intron and a neomycin resistance cassette (NEO) that was flanked by frt1 sites and was followed by a second loxP site introduced in the 3 intron. Flp recombinase removed the frt flanked neo-selection cassette. Cre recombinase removed the loxP-flanked exon 2. (J:249264)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syt7 Mutation: 42 strains or lines available

References

• Original: J:249264 Wu D, et al., Postsynaptic synaptotagmins mediate AMPA receptor exocytosis during LTP. Nature. 2017 Apr 20;544(7650):316-321
• All: 2 reference(s)