Gt(ROSA)26Sor^{tm1(Sirt6)Mpgu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Sirt6)Mpgu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mahesh P Gupta
• MGI ID: MGI:7525638
• Synonyms: ROSA26-Sirt6-FLAG, Sk.T6Tg, Tg.Sirt6
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Sirt6)Mpgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338782
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Sirt6)Mpgu} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sirt6 (MGI:1354161)

• Mutation details: The targeting vector was designed to insert the Sirt6-FLAG cDNA into the Gt(ROSA)26Sor driven by its promoter and controlled by a STOP cassette. The STOP cassette in the vector consisted of a splice acceptor and the floxed (PGK)/gb2neoPGKpolyA2xSV40pA sequence and was inserted in intron 1 of ROSA26 locus. Mouse Sirt6 cDNA with a C-terminal FLAG tag was cloned immediately downstream of the STOP cassette. The Sirt6-FLAG sequence was followed by a BGHpA bovine growth hormone polyadenylation (BGHpA) sequence. (J:338782)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:338782 Kanwal A, et al., The nuclear and mitochondrial sirtuins, Sirt6 and Sirt3, regulate each other's activity and protect the heart from developing obesity-mediated diabetic cardiomyopathy. FASEB J. 2019 Oct;33(10):10872-10888
• All: 3 reference(s)