Fabp4^tm1.1Hota
Targeted Allele Detail

Summary

• Symbol: Fabp4^tm1.1Hota
• Name: fatty acid binding protein 4, adipocyte; targeted mutation 1.1, Gokhan S Hotamisligil
• MGI ID: MGI:7525599
• Synonyms: Fabp4-floxed
• Gene: Fabp4 Location: Chr3:10269406-10273653 bp, - strand Genetic Position: Chr3, 2.56 cM
• Alliance: Fabp4^tm1.1Hota page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339978
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The murine Fabp4 sequence was humanized by introducing point mutations to convert the 11 amino acids in exons 2 and 3 that differ between mouse and human FABP4 to human FABP4 sequences. A loxP site was inserted within intron 1 upstream of exon 2 and a FRT-neomycin-FRT-loxP cassette was inserted at the 3 end of exon 2. Resulted chimera were crossed to Flp recombinaseexpressing mice to excise neomycin selection cassette. (J:339978)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fabp4 Mutation: 44 strains or lines available

References

• Original: J:339978 Inouye KE, et al., Endothelial-derived FABP4 constitutes the majority of basal circulating hormone and regulates lipolysis-driven insulin secretion. JCI Insight. 2023 Jul 24;8(14)
• All: 1 reference(s)