Mmp14^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mmp14^{tm1d(EUCOMM)Hmgu}
• Name: matrix metallopeptidase 14 (membrane-inserted); targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7525598
• Gene: Mmp14 Location: Chr14:54669055-54679913 bp, + strand Genetic Position: Chr14, 27.79 cM
• Alliance: Mmp14^{tm1d(EUCOMM)Hmgu} page
• IMPC: Mmp14 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339967
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 54672773 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 54674685. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:339967)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mmp14 Mutation: 43 strains or lines available

References

• Original: J:339967 Xia XD, et al., Global, but not chondrocyte-specific, MT1-MMP deficiency in adult mice causes inflammatory arthritis. Matrix Biol. 2023 Aug 18
• All: 1 reference(s)