Svil^em1.1Luna
Endonuclease-mediated Allele Detail

Summary

• Symbol: Svil^em1.1Luna
• Name: supervillin; endonuclease-mediated mutation 1, Elizabeth J Luna
• MGI ID: MGI:7525411
• Gene: Svil Location: Chr18:4920540-5119299 bp, + strand Genetic Position: Chr18, 3.02 cM
• Alliance: Svil^em1.1Luna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339941
• Parent Cell Line: iTL BF1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology replaced exon 3 with an ivNeo selection cassette. The ivNeo selection cassette was removed via flp-mediated recombination in the ES cells. Deletion of exon 3 results in the absence of the long muscle-specific archvillin splice-form. (J:339941)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Svil Mutation: 128 strains or lines available

References

• Original: J:339941 Smith TC, et al., Novel gamma-sarcoglycan interactors in murine muscle membranes. Skelet Muscle. 2022 Jan 22;12(1):2
• All: 1 reference(s)