Prdm9^em1Bdm
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prdm9^em1Bdm
• Name: PR domain containing 9; endonuclease-mediated mutation 1, Bernard de Massy
• MGI ID: MGI:7523944
• Synonyms: Prdm9^deltaKB1
• Gene: Prdm9 Location: Chr17:15763341-15784616 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Prdm9^em1Bdm page

Mutation origin

• Strain of Origin: CBA

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 26-bp deletion in the KRAB domain. This deletion leads to a frameshift and a protein that is translated from an internal AUG codon and lacks the first 42 amino acids, including several conserved residues of the KRAB A domain, which was confirmed in testis protein extracts by Western blot analysis. Protein levels of the N-terminal truncated protein are decreased in homozygous and heterozygous protein extracts (J:320904)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm9 Mutation: 45 strains or lines available

References

• Original: J:320904 Imai Y, et al., The PRDM9 KRAB domain is required for meiosis and involved in protein interactions. Chromosoma. 2017 Dec;126(6):681-695
• All: 1 reference(s)