Myf5^tm1.1Pstu
Targeted Allele Detail

Summary

• Symbol: Myf5^tm1.1Pstu
• Name: myogenic factor 5; targeted mutation 1.1, Pascal Stuelsatz
• MGI ID: MGI:7521969
• Synonyms: Myf5-EwCrimson, Myf5^{tm3956(T2A-E2Crimson)Tac}
• Gene: Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
• Alliance: Myf5^tm1.1Pstu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339277
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contains the Myf5 open reading frame (ORF), a Kozak sequence to allow protein translation initiation. A T2A self-cleaving peptide, E2Crimson fluorescent reporter sequence, and 3 nuclear localization signals (3xNLS) inserted after exon 3, just upstream of the 3' untranslated region (UTR), of the Myf5 gene. An FRT-flanked puromycin selection cassette was also included after the 3' UTR. Flp-mediated recombination removed the puromycin cassette. (J:339277)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myf5 Mutation: 17 strains or lines available

References

• Original: J:339277 Ancel S, et al., A Dual Color Pax7 and Myf5 In Vivo Reporter to Investigate Muscle Stem Cell Heterogeneity in Regeneration and Aging. bioRxiv. 2023
• All: 1 reference(s)