Otulin^{tm2e.1(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Otulin^{tm2e.1(EUCOMM)Hmgu}
• Name: OTU deubiquitinase with linear linkage specificity; targeted mutation 2e.1, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7521963
• Synonyms: Otulin^deltaex3
• Gene: Otulin Location: Chr15:27606005-27630793 bp, - strand Genetic Position: Chr15, 10.31 cM
• Alliance: Otulin^{tm2e.1(EUCOMM)Hmgu} page
• IMPC: Otulin gene page

Mutation origin

• Mutant Cell Lines: HEPD0595_8_C10, HEPD0595_8_G07
• Germline Transmission: Earliest citation of germline transmission: J:339280
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 27620175 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression removed the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:339280)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otulin Mutation: 22 strains or lines available

References

• Original: J:339280 Almeida SM, et al., An interaction between OTULIN and SCRIB uncovers roles for linear ubiquitination in planar cell polarity. Dis Model Mech. 2023 Aug 1;16(8)
• All: 1 reference(s)