Pole^em1Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pole^em1Tcp
• Name: polymerase (DNA directed), epsilon; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:7520417
• Synonyms: Pole^S459F
• Gene: Pole Location: Chr5:110434185-110485319 bp, + strand Genetic Position: Chr5, 53.45 cM, cytoband E3-E5
• Alliance: Pole^em1Tcp page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: This allele was generated at The Centre for Phenogenomics by injecting Cas9 mRNA with a guide RNA with the spacer sequence AGCATCTGACACTGAGTAAGand a single-strand oligonucleotide encoding the changes c.1376_1377CA>TC (p.S459F) and a silent mutation c.1368C>T (p.A456) to disrupt the protospacer adjacent motif (PAM) sequence. (J:339146)

Tumor Data

List all tumor models in MMHCdb carrying Pole^em1Tcp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pole Mutation: 103 strains or lines available

References

• Original: J:339146 Galati MA, et al., Cancers from Novel Pole-Mutant Mouse Models Provide Insights into Polymerase-Mediated Hypermutagenesis and Immune Checkpoint Blockade. Cancer Res. 2020;80(24):5606-5618
• All: 1 reference(s)