Aldh1b1^{tm2d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Aldh1b1^{tm2d(EUCOMM)Wtsi}
• Name: aldehyde dehydrogenase 1 family, member B1; targeted mutation 2d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7519101
• Synonyms: Aldh1b1 constitutive-knockout
• Gene: Aldh1b1 Location: Chr4:45799022-45804604 bp, + strand Genetic Position: Chr4, 24.35 cM, cytoband B2
• Alliance: Aldh1b1^{tm2d(EUCOMM)Wtsi} page
• IMPC: Aldh1b1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338916
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 45802160 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 45804115. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:338916)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aldh1b1 Mutation: 33 strains or lines available

References

• Original: J:338916 Cerretelli G, et al., Acetaldehyde and defective mismatch repair increase colonic tumours in a Lynch syndrome model with Aldh1b1 inactivation. Dis Model Mech. 2023 Aug 1;16(8)
• All: 1 reference(s)