Larp4^tm1Rjma
Targeted Allele Detail

Summary

• Symbol: Larp4^tm1Rjma
• Name: La ribonucleoprotein 4; targeted mutation 1, Richard J Maraia
• MGI ID: MGI:7518995
• Synonyms: Larpfloxed^floxed+Neo
• Gene: Larp4 Location: Chr15:99867946-99914239 bp, + strand Genetic Position: Chr15, 56.13 cM
• Alliance: Larp4^tm1Rjma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264766
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 99884799 of Chromosome 15 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 5 at position 99884867. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:264766)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Larp4 Mutation: 46 strains or lines available

References

• Original: J:264766 Mattijssen S, et al., LARP4 mRNA codon-tRNA match contributes to LARP4 activity for ribosomal protein mRNA poly(A) tail length protection. Elife. 2017 Sep 12;6:e28889
• All: 1 reference(s)