Slc6a3^tm1.1Emfu
Targeted Allele Detail

Summary

• Symbol: Slc6a3^tm1.1Emfu
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 1.1, Ernst-Martin Fuchtbauer
• MGI ID: MGI:7518352
• Synonyms: DAT-AAA
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^tm1.1Emfu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266803
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: The C-terminal PDZ-binding sequence LKV was substituted with alanines (AAA) in exon 15. Silent restriction enzyme sites were also introduced along with a loxP-flanked neomycin resistance gene upstream of exon 15. The neomycin cassette was deleted via cre-mediated recombination. Quantitative real-time PCR showed normal mRNA levels in midbrain tissue. (J:266803)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

References

• Original: J:266803 Rickhag M, et al., A C-terminal PDZ domain-binding sequence is required for striatal distribution of the dopamine transporter. Nat Commun. 2013;4:1580
• All: 2 reference(s)