Ahsp^tm2.1Mjwe
Targeted Allele Detail

Summary

• Symbol: Ahsp^tm2.1Mjwe
• Name: alpha hemoglobin stabilizing protein; targeted mutation 2.1, Mitchell J Weiss
• MGI ID: MGI:7517218
• Synonyms: Ahsp^P30A
• Gene: Ahsp Location: Chr7:127902016-127902926 bp, + strand
• Alliance: Ahsp^tm2.1Mjwe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338784
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Proline codon 30 (CCT) in exon 3 was changed to alanine (GCT) (p.P30A) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 2. The neo cassette was removed through subsequent Cre-mediated recombination. The affected residue is located in loop 1, between helix 1 and 2, of the encoded peptide and is thought to be involved in oxygenated alpha-globin binding. (J:338784)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ahsp Mutation: 1 strain or line available

References

• Original: J:338784 Khandros E, et al., Insights into hemoglobin assembly through in vivo mutagenesis of alpha-hemoglobin stabilizing protein. J Biol Chem. 2012 Mar 30;287(14):11325-37
• All: 1 reference(s)