Gt(ROSA)26Sor^{tm11.1(CAG-EGFP,-tdTomato)Maoh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11.1(CAG-EGFP,-tdTomato)Maoh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11.1, Masato Ohtsuka
• MGI ID: MGI:7516496
• Synonyms: Dre reporter
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11.1(CAG-EGFP,-tdTomato)Maoh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173808
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the expression cassette between the JT15 and lox2272 sites of Gt(ROSA)26Sor^tm1Maoh with CAG promoter-rox-EGFP-poly(A)-CAT (chloramphenicol acetyltransferase)-poly(A)-rox-tdTomato-poly(A) cassette. Flp-mediated recombination removed the extra sequence flanked by FRT sites. The EGFP expressed ubiquitously from the CAG promoter switch to tdTomato expression in the cells expressing Dre recombinase. (J:173808, J:303408)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:173808 Ohtsuka M, et al., Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res. 2010 Dec 1;38(22):e198
• All: 3 reference(s)