Mapt^{tm1.1(MAPT)Hcy}
Targeted Allele Detail

Summary

• Symbol: Mapt^{tm1.1(MAPT)Hcy}
• Name: microtubule-associated protein tau; targeted mutation 1.1, Holger Cynis
• MGI ID: MGI:7516312
• Synonyms: htau-KI
• Gene: Mapt Location: Chr11:104122216-104222916 bp, + strand Genetic Position: Chr11, 67.79 cM, cytoband E1
• Alliance: Mapt^{tm1.1(MAPT)Hcy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338832
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mapt^{tm1.1(MAPT)Hcy} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAPT (4137)

  Mapt	(MGI:97180)

• Mutation details: The human tau gene exons 2-12 (from BAC RP11-111I23) flanked with loxP sites replaced the mouse genomic sequence from the translation initiation codon in exon 2 to the termination codon in exon 10. The gene Saitohin (STH) is present in the human tau gene and is included in the targeting vector. In addition, an FRT-flanked neomycin resistance cassette was inserted downstream of the first loxP site and was removed via flp-mediated recombination. Murine and human tau-specific antibodies confirmed the absence of the mouse protein and presence of the human protein, respectively. (J:338832)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapt Mutation: 453 strains or lines available

References

• Original: J:338832 Barendrecht S, et al., A novel human tau knock-in mouse model reveals interaction of Abeta and human tau under progressing cerebral amyloidosis in 5xFAD mice. Alzheimers Res Ther. 2023 Jan 14;15(1):16
• All: 1 reference(s)