Sting1^em2Nyan
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sting1^em2Nyan
• Name: stimulator of interferon response cGAMP interactor 1; endonuclease-mediated mutation 2, Nan Yan
• MGI ID: MGI:7516143
• Synonyms: Sting1^S365A
• Gene: Sting1 Location: Chr18:35866732-35873607 bp, - strand Genetic Position: Chr18, 19.23 cM, cytoband B3
• Alliance: Sting1^em2Nyan page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codon 365 (AGT) in exon 8 was changed to alanine (GCT) (p.S365A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation changes a phosphorylatable residue in the encoded peptide into a phosphoblocker, which selectively inactivates the IFN signaling activity of the protein. (J:304742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sting1 Mutation: 45 strains or lines available

References

• Original: J:304742 Wu J, et al., Interferon-Independent Activities of Mammalian STING Mediate Antiviral Response and Tumor Immune Evasion. Immunity. 2020 Jul 14;53(1):115-126.e5
• All: 2 reference(s)