Ilk^tm1.1Zentr
Targeted Allele Detail

Summary

• Symbol: Ilk^tm1.1Zentr
• Name: integrin linked kinase; targeted mutation 1.1, Roy Zent
• MGI ID: MGI:7513913
• Synonyms: E359K-ILK
• Gene: Ilk Location: Chr7:105385799-105392132 bp, + strand Genetic Position: Chr7, 55.97 cM, cytoband E1
• Alliance: Ilk^tm1.1Zentr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304821
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Glutamic acid codon 359 (GAA) in exon 11 was changed to lysine (AAA) (p.E359K) and a loxP site flanked neomycin resistance gene cassette was inserted into the 3' UTR in exon 13. The neo cassette was removed through subsequent Cre-mediated recombination. This mutation negatively affects the stability of the encoded peptide. (J:304821)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ilk Mutation: 22 strains or lines available

References

• Original: J:304821 Bulus N, et al., Disruption of the integrin-linked kinase (ILK) pseudokinase domain affects kidney development in mice. J Biol Chem. 2021 Feb 1;:100361
• All: 1 reference(s)