Chek1^tm1.1Jani
Targeted Allele Detail

Summary

• Symbol: Chek1^tm1.1Jani
• Name: checkpoint kinase 1; targeted mutation 1.1, Jonas A Nilsson
• MGI ID: MGI:7513019
• Synonyms: Chek1^D130A
• Gene: Chek1 Location: Chr9:36619935-36637897 bp, - strand Genetic Position: Chr9, 20.67 cM, cytoband A5.3
• Alliance: Chek1^tm1.1Jani page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305083
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Aspartic acid codon 130 (GAT) in exon 5 was changed to alanine (GCT) (p.D130A) and an FRT site flanked neomycin resistance gene cassette was inserted into intron 3. The neo cassette was removed through subsequent Flp-mediated recombination. The mutation changes the phosphorylatable to an un-phosphorylatable residue in the encoded peptide and renders it kinase-dead. (J:305083)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chek1 Mutation: 41 strains or lines available

References

• Original: J:305083 Muralidharan SV, et al., Small molecule inhibitors and a kinase-dead expressing mouse model demonstrate that the kinase activity of Chk1 is essential for mouse embryos and cancer cells. Life Sci Alliance. 2020 Aug;3(8)
• All: 1 reference(s)