Dot1l^tm1.2Saam
Targeted Allele Detail

Summary

• Symbol: Dot1l^tm1.2Saam
• Name: DOT1 like histone lysine methyltransferase; targeted mutation 1.2, Scott Armstrong
• MGI ID: MGI:7511880
• Gene: Dot1l Location: Chr10:80591040-80631295 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dot1l^tm1.2Saam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335601
• Parent Cell Line: CJ9 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 5. An FRT-flanked puro cassette with a 3' loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the puro cassette and left exon 5 floxed. Cre-mediated recombination removed exon 5. (J:335601)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dot1l Mutation: 75 strains or lines available

References

• Original: J:335601 Malla AB, et al., DOT1L promotes spermatid differentiation by regulating expression of genes required for histone-to-protamine replacement. Development. 2023 May 1;150(9):dev201497
• All: 1 reference(s)