Kat2b^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kat2b^{tm1b(KOMP)Wtsi}
• Name: K(lysine) acetyltransferase 2B; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7511792
• Synonyms: Kat2b KO
• Gene: Kat2b Location: Chr17:53873889-53979748 bp, + strand Genetic Position: Chr17, 27.86 cM
• Alliance: Kat2b^{tm1b(KOMP)Wtsi} page
• IMPC: Kat2b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338308
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 53930944 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 53931028. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:338308)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kat2b Mutation: 152 strains or lines available

References

• Original: J:338308 Walters BW, et al., Divergent functions of histone acetyltransferases KAT2A and KAT2B in keratinocyte self-renewal and differentiation. J Cell Sci. 2023 Jun 15;136(12):jcs260723
• All: 1 reference(s)