Bmp10^{tm3c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Bmp10^{tm3c(EUCOMM)Hmgu}
• Name: bone morphogenetic protein 10; targeted mutation 3c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7511709
• Synonyms: Bmp10^lox
• Gene: Bmp10 Location: Chr6:87405976-87411494 bp, + strand Genetic Position: Chr6, 37.96 cM, cytoband D2
• Alliance: Bmp10^{tm3c(EUCOMM)Hmgu} page
• IMPC: Bmp10 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338274
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: LoxP sites flank exon 2. The L1L2_Bact_P cassette was inserted at position 87433089 of Chromosome 6 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87434716. The critical exon(s) is/are thus flanked by loxP sites.A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:338274)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bmp10 Mutation: 18 strains or lines available

References

• Original: J:338274 Ouarne M, et al., BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer. J Exp Clin Cancer Res. 2018 Aug 30;37(1):209
• All: 3 reference(s)