Gt(ROSA)26Sor^{tm1(CAG-ACR2/EYFP)Ksak}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ACR2/EYFP)Ksak}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kenji Sakimura
• MGI ID: MGI:7511697
• Synonyms: Rosa26-CAGp-LSL-ACR2-eYFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ACR2/EYFP)Ksak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:338273
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Transgenic mice carry a construct knocked into the Gt(ROSA)26Sor locus, consisting of a CAG promoter, and frt-flanked pgk-Neo cassette, a STOP cassette consisting of the terminator of the yeast His3 gene and SV40 poly-adenylation sequence37, cDNA encoding fluorescent Guillardia theta microbial rhodopsin ACR2 cDNA tagged with EYFP at the C-terminus, a woodchuck hepatitis virus post-transcriptional regulatory element, and the rabbit beta-globin poly-adenylation sequence. Two loxP sites were inserted before the frt-Neo cassette and after the STOP cassette. (J:338273)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:338273 Mukai Y, et al., Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity. Sci Rep. 2023 Mar 9;13(1):3966
• All: 1 reference(s)