Fgg^tm1.1Geno
Targeted Allele Detail

Summary

• Symbol: Fgg^tm1.1Geno
• Name: fibrinogen gamma chain; targeted mutation 1.1, Genoway
• MGI ID: MGI:7511552
• Synonyms: FGG3X
• Gene: Fgg Location: Chr3:82915031-82922356 bp, + strand Genetic Position: Chr3, 36.94 cM
• Alliance: Fgg^tm1.1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307658
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: Glutamine codons 423 and 424 (CAG) in exon 9 were changed to asparagine (p.Q423N, p.Q424N), lysine codon 431 (AAA) to arginine (p.K431R) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 8. The neo cassette was removed through subsequent Cre-mediated recombination. The mutations eliminate the gamma-chain cross-linking sites and recapitulate the same experimentally created mutations in the human ortholog (p.Q398N p.Q399N, p.K406R in the mature peptide) that affect blood clot formation. (J:307658)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgg Mutation: 29 strains or lines available

References

• Original: J:307658 Duval C, et al., Elimination of fibrin gamma-chain cross-linking by FXIIIa increases pulmonary embolism arising from murine inferior vena cava thrombi. Proc Natl Acad Sci U S A. 2021 Jul 6;118(27):e2103226118
• All: 1 reference(s)