Col1a1^{tm6.1(tetO-Mycn,-mCherry)Yasu}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm6.1(tetO-Mycn,-mCherry)Yasu}
• Name: collagen, type I, alpha 1; targeted mutation 6.1, Yasuhiro Yamada
• MGI ID: MGI:7509704
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm6.1(tetO-Mycn,-mCherry)Yasu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330715
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm6.1(tetO-Mycn,-mCherry)Yasu} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mycn (MGI:97357)

• Mutation details: RMCE knocked in the construct containing a tetracycline responsive element driving inducible expression of the Mycn with an IRES sequence and an mCherry reporter gene into the 3' UTR. The blasticidin S selection cassette was removed by a vector expressing Dre recombinase. (J:330715)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:330715 Hirano M, et al., MYCL-mediated reprogramming expands pancreatic insulin-producing cells. Nat Metab. 2022;4(2):254-268
• All: 1 reference(s)