Smg7^tm1.1Tany
Targeted Allele Detail

Summary

• Symbol: Smg7^tm1.1Tany
• Name: SMG7 nonsense mediated mRNA decay factor; targeted mutation 1.1, Yi Tang
• MGI ID: MGI:7509491
• Synonyms: Smg7^flox, Smg7^tm1c
• Gene: Smg7 Location: Chr1:152712746-152778397 bp, - strand Genetic Position: Chr1, 65.07 cM
• Alliance: Smg7^tm1.1Tany page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:336915
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting cassette was inserted upstream of the critical exon 2. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. The fisrt loxP site is followed by a neomycin resistance gene under the control of the beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2. The critical exon 2 is thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination leaving exon 2 floxed. (J:336915)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smg7 Mutation: 70 strains or lines available

References

• Original: J:336915 Steiner AJ, et al., Characterization of a rhabdomyosarcoma reveals a critical role for SMG7 in cancer cell viability and tumor growth. Sci Rep. 2023 Jun 22;13(1):10152
• All: 1 reference(s)