B4galnt3^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: B4galnt3^{tm1d(EUCOMM)Wtsi}
• Name: beta-1,4-N-acetyl-galactosaminyl transferase 3; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7509340
• Gene: B4galnt3 Location: Chr6:120180034-120271520 bp, - strand Genetic Position: Chr6, 56.94 cM
• Alliance: B4galnt3^{tm1d(EUCOMM)Wtsi} page
• IMPC: B4galnt3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337847
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 120198699 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 120199784. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:337847)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any B4galnt3 Mutation: 45 strains or lines available

References

• Original: J:337847 Moverare-Skrtic S, et al., B4GALNT3 regulates glycosylation of sclerostin and bone mass. EBioMedicine. 2023 May;91:104546
• All: 1 reference(s)