Trem2^em1Npa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Trem2^em1Npa
• Name: triggering receptor expressed on myeloid cells 2; endonuclease-mediated mutation 1, Novartis Pharma AG
• MGI ID: MGI:7507636
• Synonyms: Trem2-IPD
• Gene: Trem2 Location: Chr17:48653429-48659304 bp, + strand Genetic Position: Chr17, 23.99 cM, cytoband C
• Alliance: Trem2^em1Npa page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated a CACAGCACC to ATCCCCGAC change, resulting in the substitution of amino acids histidine 157, serine 158 and threonine 159 (HST) to isoleucine, proline, and aspartate (IPD). IPD are the least-preferred amino acids for the sub-site biding of ADAM10/17 and this change is expected to reduce TREM2 proteolytic cleavage. (J:337819)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trem2 Mutation: 65 strains or lines available

References

• Original: J:337819 Dhandapani R, et al., Sustained Trem2 stabilization accelerates microglia heterogeneity and Abeta pathology in a mouse model of Alzheimer's disease. Cell Rep. 2022 May 31;39(9):110883
• All: 2 reference(s)