Arhgef18^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Arhgef18^{tm1c(KOMP)Mbp}
• Name: Rho/Rac guanine nucleotide exchange factor 18; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:7506590
• Gene: Arhgef18 Location: Chr8:3403415-3506601 bp, + strand Genetic Position: Chr8, 1.92 cM
• Alliance: Arhgef18^{tm1c(KOMP)Mbp} page
• IMPC: Arhgef18 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307822
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 3486491 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 3486562. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:307822)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Arhgef18 Mutation: 241 strains or lines available

References

• Original: J:307822 Beal R, et al., ARHGEF18/p114RhoGEF Coordinates PKA/CREB Signaling and Actomyosin Remodeling to Promote Trophoblast Cell-Cell Fusion During Placenta Morphogenesis. Front Cell Dev Biol. 2021;9:658006
• All: 2 reference(s)