Mark3^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Mark3^{tm1b(KOMP)Mbp}
• Name: MAP/microtubule affinity regulating kinase 3; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:7506569
• Gene: Mark3 Location: Chr12:111540957-111622655 bp, + strand Genetic Position: Chr12, 61.05 cM, cytoband F2
• Alliance: Mark3^{tm1b(KOMP)Mbp} page
• IMPC: Mark3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305797
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 111589255 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 111589283. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:305797)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mark3 Mutation: 71 strains or lines available

References

• Original: J:305797 Zhang Q, et al., Genomic variants within chromosome 14q32.32 regulate bone mass through MARK3 signaling in osteoblasts. J Clin Invest. 2021 Apr 1;131(7)
• All: 1 reference(s)