Tmem106b^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem106b^{tm2c(KOMP)Wtsi}
• Name: transmembrane protein 106B; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7506498
• Synonyms: Tmem106b^flox
• Gene: Tmem106b Location: Chr6:13069758-13089268 bp, + strand Genetic Position: Chr6, 5.38 cM, cytoband A2
• Alliance: Tmem106b^{tm2c(KOMP)Wtsi} page
• IMPC: Tmem106b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337780
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 13074587 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 13074664. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:337780)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem106b Mutation: 29 strains or lines available

References

• Original: J:337780 Zhang T, et al., TMEM106B regulates microglial proliferation and survival in response to demyelination. Sci Adv. 2023 May 5;9(18):eadd2676
• All: 2 reference(s)