Il18^em1Cga
Endonuclease-mediated Allele Detail

Summary

• Symbol: Il18^em1Cga
• Name: interleukin 18; endonuclease-mediated mutation 1, Cem Gabay
• MGI ID: MGI:7506220
• Synonyms: IL-18 KO Neongreen reporter
• Gene: Il18 Location: Chr9:50466127-50493140 bp, + strand Genetic Position: Chr9, 27.75 cM
• Alliance: Il18^em1Cga page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate an insertion of a codon optimized 3x FLAG tag sequence followed by SV40-NLS sequence, an mNeongreen sequence followed by an SV40-NLS and 6-His tag sequences. After selection of guide RNAs as closest as possible to the IL-18 gene start and stop codons, all IL-18 sequences between start and stop codon (exons 2-6) were replaced by the mNeongreen cassette. (J:337717)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il18 Mutation: 31 strains or lines available

References

• Original: J:337717 Fauteux-Daniel S, et al., The role of interleukin-18 and interleukin-18 binding protein in K/BxN serum transfer-induced arthritis. Front Immunol. 2023;14:1215364
• All: 1 reference(s)