Ift74^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ift74^{tm1d(EUCOMM)Hmgu}
• Name: intraflagellar transport 74; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7495512
• Synonyms: Ift74^Tm1d
• Gene: Ift74 Location: Chr4:94502728-94581466 bp, + strand Genetic Position: Chr4, 43.34 cM, cytoband C5
• Alliance: Ift74^{tm1d(EUCOMM)Hmgu} page
• IMPC: Ift74 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337473
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 94509640 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 94510575. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:337473)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ift74 Mutation: 42 strains or lines available

References

• Original: J:337473 Bakey Z, et al., IFT74 variants cause skeletal ciliopathy and motile cilia defects in mice and humans. PLoS Genet. 2023 Jun;19(6):e1010796
• All: 1 reference(s)