Il3^tm1.2Ics
Targeted Allele Detail

Summary

• Symbol: Il3^tm1.2Ics
• Name: interleukin 3; targeted mutation 1.2, Mouse Clinical Institute
• MGI ID: MGI:7494106
• Synonyms: Il3^L2, Il3^tm1c
• Gene: Il3 Location: Chr11:54156129-54158103 bp, - strand Genetic Position: Chr11, 32.13 cM, cytoband B1
• Alliance: Il3^tm1.2Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:336657
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/cN

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: IMPC targeting vector ETPG00275_W_2_F02 was modified to remove the lacZ and insert EGFP cDNA. The resulting vector contains an FRT site, En2 splice acceptor, LF2A sequence, EGFP, loxP site, neomycin resistance cassette, FRT site, and loxP site were inserted into intron 2 and third lox P site was inserted downstream of exon 5. The EGFP and neomycin resistance cassette were removed via flp-mediated recombination leaving exons 3-5 floxed. (J:336657)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il3 Mutation: 30 strains or lines available

References

• Original: J:336657 Hachem CE, et al., IL-3 produced by T cells is crucial for basophil extravasation in hapten-induced allergic contact dermatitis. Front Immunol. 2023;14:1151468
• All: 1 reference(s)