Col1a1^{tm1(tetO-cas9*)Ldow}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-cas9*)Ldow}
• Name: collagen, type I, alpha 1; targeted mutation 1, Lukas E Dow
• MGI ID: MGI:7493844
• Synonyms: iBE, TRE-BE3RA
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-cas9*)Ldow} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337058
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Endonuclease, Inducible)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert an expression optimized iBE reporter construct downstream of the Col1a1 locus. The construct contains a tetO promoter, an optimized BE3RA and rabbit beta globin polyA (RBGpA) sequence. BE3RA contains a rat APOBEC cytidine deaminase at the N-terminus of inactive dCas9 (Cas9-D10A) and a uracil glycosylase inhibitor (UGI) domain at the C-terminus. (J:337058)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

References

• Original: J:337058 Katti A, et al., Rapid generation of precision preclinical cancer models using regulatable in vivo base editing. bioRxiv. 2022
• All: 1 reference(s)