Slc35f1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc35f1^{tm1b(KOMP)Wtsi}
• Name: solute carrier family 35, member F1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7493842
• Gene: Slc35f1 Location: Chr10:52566629-52987718 bp, + strand Genetic Position: Chr10, 26.79 cM
• Alliance: Slc35f1^{tm1b(KOMP)Wtsi} page
• IMPC: Slc35f1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337057
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 52808642 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 52808736. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:337057)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc35f1 Mutation: 29 strains or lines available

References

• Original: J:337057 Ehlers JS, et al., Morphological and behavioral analysis of Slc35f1-deficient mice revealed no neurodevelopmental phenotype. Brain Struct Funct. 2023 May;228(3-4):895-906
• All: 1 reference(s)