Rab35^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rab35^{tm1c(EUCOMM)Hmgu}
• Name: RAB35, member RAS oncogene family; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7493678
• Synonyms: Rab35^flox
• Gene: Rab35 Location: Chr5:115769953-115785217 bp, + strand Genetic Position: Chr5, 56.1 cM
• Alliance: Rab35^{tm1c(EUCOMM)Hmgu} page
• IMPC: Rab35 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335166
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 115777657 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 115778534. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:335166)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab35 Mutation: 19 strains or lines available

References

• Original: J:335166 Maejima I, et al., RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution. Commun Biol. 2023 Apr 21;6(1):440
• All: 2 reference(s)