Aplp2^{tm2c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Aplp2^{tm2c(EUCOMM)Hmgu}
• Name: amyloid beta precursor-like protein 2; targeted mutation 2c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7493665
• Synonyms: Aplp2^fl, APLP2^tm1c
• Gene: Aplp2 Location: Chr9:31060853-31123111 bp, - strand Genetic Position: Chr9, 16.66 cM, cytoband A2-B
• Alliance: Aplp2^{tm2c(EUCOMM)Hmgu} page
• IMPC: Aplp2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:307755
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31082596 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 31083716. The critical exon(s) is/are thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:307755)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aplp2 Mutation: 30 strains or lines available

References

• Original: J:307755 Poelaert BJ, et al., Amyloid Precursor-like Protein 2 Expression Increases during Pancreatic Cancer Development and Shortens the Survival of a Spontaneous Mouse Model of Pancreatic Cancer. Cancers (Basel). 2021 Mar 26;13(7)
• All: 1 reference(s)