Cd79b^tm1.1Jagrm
Targeted Allele Detail

Summary

• Symbol: Cd79b^tm1.1Jagrm
• Name: CD79B antigen; targeted mutation 1.1, Jagan R Muppidi
• MGI ID: MGI:7491840
• Synonyms: Cd79b^Y195H
• Gene: Cd79b Location: Chr11:106202167-106205388 bp, - strand Genetic Position: Chr11, 68.89 cM
• Alliance: Cd79b^tm1.1Jagrm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330627
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NCr

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 2 and a STOP cassette, FRT site flanked neomycin resistance gene cassette, a second loxP site and a copy of the genomic sequence from the end of intron 2 through exon 6 (with a modified exon 5) were inserted downstream of the gene. The modification in the inserted exon 5 copy entails changing tyrosine codon 195 (TAT) to histidine (CAT) (p.Y195H). This mutation, in the ITAM motif of the encoded peptide, is the equivalent of the human p.Y196H mutation associated with diffuse large B-cell lymphoma (DLBCL). The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wild-type transcript/peptide and only after Cre-mediated deletion of the endogenous exons and STOP cassette will it express the modified transcript/peptide. (J:330627)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd79b Mutation: 36 strains or lines available

References

• Original: J:330627 Pindzola GM, et al., Aberrant expansion of spontaneous splenic germinal centers induced by hallmark genetic lesions of aggressive lymphoma. Blood. 2022 Sep 8;140(10):1119-1131
• All: 1 reference(s)