Dync1h1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dync1h1^{tm1c(KOMP)Wtsi}
• Name: dynein cytoplasmic 1 heavy chain 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7491690
• Synonyms: Dync1h1^F
• Gene: Dync1h1 Location: Chr12:110567886-110633379 bp, + strand Genetic Position: Chr12, 60.7 cM
• Alliance: Dync1h1^{tm1c(KOMP)Wtsi} page
• IMPC: Dync1h1 gene page

Mutation origin

• Mutant Cell Line: EPD0284_5_D04
• Germline Transmission: Earliest citation of germline transmission: J:336670
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 110596617 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 110596632. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:336670)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dync1h1 Mutation: 197 strains or lines available

References

• Original: J:336670 Di Pizio A, et al., A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensing. J Cell Sci. 2023 Mar 1;136(5)
• All: 4 reference(s)