Kcnt1^em1Trsg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kcnt1^em1Trsg
• Name: potassium channel, subfamily T, member 1; endonuclease-mediated mutation 1, Tracy S Gertler
• MGI ID: MGI:7490247
• Synonyms: Kcnt1-L437F
• Gene: Kcnt1 Location: Chr2:25753807-25808285 bp, + strand Genetic Position: Chr2, 18.27 cM
• Alliance: Kcnt1^em1Trsg page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: Leucine codon 437 (CTC) in exon 14 was changed to phenylalanine (TTC) (NM_175462.4:c.1309C>T, p.L437F) using an sgRNA (targeting GACCTCATTCCTGCTGCTG) and an ssODN template with CRISPR/Cas9 technology. This gain-of-function mutation of the highly conserved leucine in the RCK1 domain of the encoded peptide is the equivalent of the human p.L456F mutation. (J:324580)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnt1 Mutation: 70 strains or lines available

References

• Original: J:324580 Gertler TS, et al., KNa1.1 gain-of-function preferentially dampens excitability of murine parvalbumin-positive interneurons. Neurobiol Dis. 2022 Jun 15;168:105713
• All: 1 reference(s)