Vps13c^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Vps13c^{tm1d(EUCOMM)Hmgu}
• Name: vacuolar protein sorting 13C; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7489965
• Synonyms: Vps13c^tm1d
• Gene: Vps13c Location: Chr9:67747678-67902920 bp, + strand Genetic Position: Chr9, 36.77 cM
• Alliance: Vps13c^{tm1d(EUCOMM)Hmgu} page
• IMPC: Vps13c gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:336507
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 67760530 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 67761274. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:336507)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vps13c Mutation: 168 strains or lines available

References

• Original: J:336507 Hancock-Cerutti W, et al., ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. J Cell Biol. 2022 Jul 4;221(7):e202106046
• All: 2 reference(s)