Dicer1^em4Psv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dicer1^em4Psv
• Name: dicer 1, ribonuclease type III; endonuclease-mediated mutation 4, Petr Svoboda
• MGI ID: MGI:7489654
• Synonyms: Dicer1^SOM
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^em4Psv page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Endogenous exons 2-6 and the LTR insertions in intron 6 were deleted, and a construct containing an HA-tag at the N-terminus of the coding sequence and DNA sequence of exon 2 to exon 7 was inserted. This eliminated the expression of a truncated Dicer isoform in mouse oocytes that is dependent on the LTR insertions. (J:285120)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:285120 Taborska E, et al., Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes. PLoS Genet. 2019 Dec;15(12):e1008261
• All: 3 reference(s)