Gt(ROSA)26Sor^{em2(CAG-Mecom)Suda}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-Mecom)Suda}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Toshio Suda
• MGI ID: MGI:7488474
• Synonyms: ROSA^{CAG-Evi1-IRES-GFP}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-Mecom)Suda} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:336140
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: Using the CRISPR/Cas9 method, a targeting construct consisting of a CAG-loxP/STOP/loxP-murine Mecom cDNA followed by IRES-EGFP-pA fusion gene inserted into intron 1 of the ROSA26 locus into the Ai9 plasmid, was injected into 6NK7 embryonic stem cells. Mecom and EGFP are expressed after recombination by Cre recombinase driven by the CAG promoter. The lines containing this allele showed a high recombination rate. (J:336140)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

Notes

6NK7 embryonic stem cells are used

References

• Original: J:336140 Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
• All: 1 reference(s)