Gt(ROSA)26Sor^{em1(Mecom)Suda}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(Mecom)Suda}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Toshio Suda
• MGI ID: MGI:7488473
• Synonyms: ROSA^{Evi1-IRES-GFP}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(Mecom)Suda} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:336140
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: Using the CRISPR/Cas9 method, a targeting construct consisting of an AS-loxP/NeoSTOP/loxP-murine Mecom cDNA followed by IRES-EGFP-pA fusion gene inserted into intron 1 of the ROSA26 locus into STOP-eGFP-ROSA26TV plasmid was injected into 6NK7 embryonic stem cells. Mecom and EGFP are expressed after recombination by Cre recombinase driven by endogenous Gt(ROSA)26Sor promoter. The lines containing this allele showed a low recombination rate. (J:336140)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1061 strains or lines available

Notes

6NK7 embryonic stem cells are used

References

• Original: J:336140 Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
• All: 1 reference(s)