Nckap1l^tm1Hro
Targeted Allele Detail

Summary

• Symbol: Nckap1l^tm1Hro
• Name: NCK associated protein 1 like; targeted mutation 1, Robert A Hromas
• MGI ID: MGI:7485932
• Synonyms: Hem-1^-
• Gene: Nckap1l Location: Chr15:103362221-103407237 bp, + strand Genetic Position: Chr15, 58.96 cM
• Alliance: Nckap1l^tm1Hro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266257
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ-neo cassette, in which the neomycin phosphotransferase gene is linked to the lacZ gene placed between the independent ribosomal entry sequences and an SV40 polyadenylation signal, replaced the coding sequence of the first exon. Western blot analysis confirmed absence of protein in E14.5 fetal liver CD45+/Lin- cells. (J:266257)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nckap1l Mutation: 52 strains or lines available

References

• Original: J:266257 Shao L, et al., The Wave2 scaffold Hem-1 is required for transition of fetal liver hematopoiesis to bone marrow. Nat Commun. 2018 Jun 18;9(1):2377
• All: 2 reference(s)